Amino Acid Incorporation by Isolated Rat Liver Mitochondria during Liver Regeneration
نویسندگان
چکیده
منابع مشابه
Amino acid incorporation by isolated rat liver mitochondria during liver regeneration.
Intact mitochondria, isolated from regenerating rat liver 2-3 days after partial hepatectomy, are 2.5-3 times more active in amino acid incorporation than mitochondria from control livers. Liver mitochondria from sham-operated animals showed normal amounts of incorporation. Sterile procedures insured low levels of bacterial contamination; cycloheximide was used to eliminate any contribution by ...
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Isolated rat liver mitochondria can incorporate tritiated thymidine into DNA. The addition of deoxyribonucleotides and ATP are necessary. The process of incorporation was found to be inhibited to the extent of 92% by actinomycin D and only 14% by pancreatic deoxyribonuclease. The identification of the labeled product as mitochondrial DNA is supported by its insolubility in acid, resistance to a...
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1. Normal and thyroidectomized rats were treated with near-physiological doses of tri-iodothyronine. Liver mitochondria were isolated and incubated with radioactive amino acids. In normal rats tri-iodothyronine caused only a slight stimulation of incorporation into mitochondrial protein, but in thyroidectomized animals the incorporation was doubled. 2. There was a lag period of about 36 hr. aft...
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The data presented in this paper emphasize the importance of eliminating bacterial contamination in studies of amino acid incorporation into isolated mitochondria. A correlation between the number of bacteria present and amino acid incorporation into mitochondria was observed when pure cultures of bacteria were added to mitochondria prepared under sterile conditions. Low concentrations of dinit...
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Several major protein components of the inner membrane were isolated from rat liver mitochondria after an incubation with (14C)leucine. No significant radioactivity was present in either the fraction containing the oligomycin-sensitive ATPase or that containing cytochromes b and cl. A variable amount of radioactive label was associated with a membranous preparation of cytochrome oxidase. After ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1970
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.67.4.1695